Combi Flash 7650 Series Uživatelský manuál stáhnout pdf (Strana 149)

Section 6 Maintenance

6-5

Figure 6-1 Default post-separation column and flow cell flush

Figure 6-2 Flow cell cleaning message

Typically, chemists Stop and then Fast Forward the run after

the last compound elutes. This action skips the

post-separation flush. If any of the above conditions appear,

consider allowing some of the runs to continue through the

flush, or run a high percentage of %B solvent through the

system for a few minutes at the end of each day.

If the separation runs always continue through the flush and

the conditions still occur frequently, edit the default column

methods to extend the flush duration.

100

Gradient % Solvent B

Time or Column Volumes

Initial %B

Next Run Initial %B

End of

Separation

End of

Run

Flush

Separation

Gradient

1 2 ... 144 145 146 147 148 149 150 151 152 153 154 ... 158 159

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Combi Flash 7650 Series Uživatelský manuál Strana 149